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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which ends up in chromosome instability, and therefore may lead to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two additional splice variants were being claimed but in-depth nucleotide sequence information is not really nonetheless readily available.
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Blocking peptides are peptides that bind specially to the concentrate on antibody and block antibody binding. These peptide usually is made up of the epitope identified from the antibody.
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This manual outlines more probable optimization of mobile and immunoassay parameters to make sure the very best final results are...
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This guide outlines even further possible optimization of mobile and immunoassay parameters to be certain the absolute best success are...
Antibodies bound to the blocking peptide no more bind into the epitope within the focus on protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Goal Details Cyclin E belongs to your really conserved cyclin family members, whose customers are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinct expression and degradation patterns which add to the temporal coordination of each and every mitotic celebration. Cyclin E varieties a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S stage boundary which is degraded as cells development through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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